capB [2019-12-16 15:35:55]

capsular polyglutamate biosynthesis

Locus: BSU_35900

Isoelectric point: 5.07

Molecular weight: 43.79 kDa

Protein length: 393 aa

Gene length: 1182 bp

Function: capsule synthesis

Product: poly-gamma-glutamate synthetase

Essential: no

Synonyms: ywsC,pgsB

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Capsule biosynthesis and degradation

Gene

Coordinates: 3,699,446 3,700,627

Phenotypes of a mutant

• no synthesis of the poly-gamma-glutamate capsule PubMed

The protein

Catalyzed reaction/ biological activity

• synthesis of poly-gamma-glutamate from L-Glu PubMed

Expression and Regulation

Operons

• Genes: capB-capC-capA-capE
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • DegU: activation, DegU-P, PubMed, in DegU regulon

  Additional information

  • expression of the operon is increased in 'motA' or 'motB' mutants due to increased DegU phosphorylation PubMed

Biological materials

Mutant

• MGNA-B262 (ywsC::erm), available at the NBRP B. subtilis, Japan
• BKE35900 (capB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTGCTTTTCGACATCTCC, downstream forward: _UP4_GTAAGCTAGGGGGAAATGCA
• BKK35900 (capB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTGCTTTTCGACATCTCC, downstream forward: _UP4_GTAAGCTAGGGGGAAATGCA

References

Reviews

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Original publications

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